Genetic evaluation of childhood acute lymphoblastic leukemia in Iraq using FTA cards.

نویسندگان

  • Lika'a Fasih Y Al-Kzayer
  • Kazuo Sakashita
  • Kazuyuki Matsuda
  • Salma Abbas Al-Hadad
  • Mazin Faisal Al-Jadiry
  • Wisam Majeed Abed
  • Jaafar M H Abdulkadhim
  • Tariq Abadi Al-Shujairi
  • Janan Ghalib Hasan
  • Hussam M Salih Al-Abdullah
  • Mouroge H Al-Ani
  • Paiman Ali I Saber
  • Toshi Inoshita
  • Minoru Kamata
  • Kenichi Koike
چکیده

BACKGROUND Genetic examination of childhood leukemia has not been available in Iraq. We here report the frequency of TEL-AML1, E2A-PBX1, MLL-AF4, and BCR-ABL chimeric transcripts in 264 Iraqi children newly diagnosed with acute lymphoblastic leukemia (ALL), using FTA cards impregnated with bone marrow aspirate or whole blood. PATIENTS AND METHODS The diagnosis of ALL was made according to standard French-American-British morphologic criteria. Based on the results of storage temperature and duration, most of the FTA samples were preserved at 4°C for up to 6 weeks in five Iraqi hospitals and then transferred to Japan for molecular analysis. Nested reverse transcription-polymerase chain reaction was adopted for the analysis. RESULTS TEL-AML1 chimeric transcript product was found in 32 (12.1%) of 264 ALL patients. Eleven (4.2%) patients, 4 (1.5%) patients, and 11 (4.2%) patients had E2A-PBX1 mRNA, MLL-AF4 mRNA, and BCR-ABL mRNA, respectively. One patient had both TEL-AML1 and E2A-PBX1 fusion genes. The incidence of TEL-AML1 in Iraqi ALL children appears to be similar to or slightly higher than those of Jordan (12%) and Kuwait (7%). The prevalence and clinical findings of ALL patients with either E2A-PBX1 or BCR-ABL were comparable to the data reported elsewhere. CONCLUSION International collaboration via FTA cards may be helpful to improve diagnosis and management of patients with hematological malignancies in low-income and underdeveloped countries.

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عنوان ژورنال:
  • Pediatric blood & cancer

دوره 59 3  شماره 

صفحات  -

تاریخ انتشار 2012